Nanofilter array chip for fast gel-free biomolecule separation
نویسندگان
چکیده
منابع مشابه
A Nanofilter Array Chip for Fast Gel-Free Biomolecule Separation.
We report here a microfabricated nanofilter array chip that can size-fractionate SDS-protein complexes and small DNA molecules based on the Ogston sieving mechanism. Nanofilter arrays with a gap size of 40-180nm were fabricated and characterized. Complete separation of SDS-protein complexes and small DNA molecules were achieved in several minutes with a separation length of 5mm. The fabrication...
متن کاملA Patterned Anisotropic nanofilter Array for continuous-flow Separation of DnA and Proteins
Microfabricated regular sieving structures hold great promise as an alternative to gels to improve biomolecule separation speed and resolution. In contrast to the disordered gel porous network, these regular structures also provide well-defined environments ideal for study of molecular dynamics in confining spaces. However, previous regular sieving structures have been limited for separation of...
متن کاملHigh-throughput, Continuous-flow Separation of Biomolecules in a High- aspect-ratio Nanofilter Array
We have developed a novel fabrication ap proach to generate massively-parallel, highaspect-ratio vertical nanofluidic channels with smooth, vertical sidewalls and precise control of uniform gap sizes (lateral trench width) down to 50 nm (Figure 1) [1]. The aspect ratio can be as high as 400 and the channel depths are more than 20 μm. This technique enables us to fabricate a large area of solid ...
متن کاملAbstract Submitted for the MAR06 Meeting of The American Physical Society Sorting Category: 10.14.12 (E) Patterned Periodic Nanofilter Array for Continuous- Flow Bimolecular Separation
Submitted for the MAR06 Meeting of The American Physical Society Sorting Category: 10.14.12 (E) Patterned Periodic Nanofilter Array for ContinuousFlow Bimolecular Separation JIANPING FU, Department of Mechanical Engineering, MIT, JONGYOON HAN, Department of Electrical Engineering and Computer Science, Division of Biological Engineering, MIT — We present an experimental study on sieving process ...
متن کاملFast Methods for Biomolecule Charge Optimization
Charge optimization is an essential element of rational drug design; given ligand and receptor proteins, one wishes to determine the ligand charge distribution—a vector of partial atomic charges—that maximizes the favorable change in electrostatic free energy on binding. Work in biophysics has shown that this problem is convex, and that it can be solved using standard quadratic programming meth...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Physics Letters
سال: 2005
ISSN: 0003-6951,1077-3118
DOI: 10.1063/1.2149979